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SIBO Lactulose Breath Test Kit

SIBO Lactulose Breath Test Kit

SIBO Lactulose Breath Test Kit

SIBO can be tricky; don't stop when you're tired, stop when your fixed! 
Tips for the most accurate results:
1- You can start by clicking the link above, download and review all this information/instructions. 
2- The diet must be adhered to, you must follow a low fiber diet. It's designed that way for a reason, if you can't adhere to it, don't waste your money.
3- The breath collection device has a tiny hole within the blue bag portion. Leave it, don't tape it over, it is there for a reason.
4- Don't treat the breath collection device like it's a balloon, simple easy breaths are all that's require. 
5- Do not press/puncture the tubes too soon, allow the breath collection device to expand before you press the tubes down. Give the tube a good push, hold for approximately 2 seconds then pull them back out. Your breath is captured. 
6- Inside the breath collection device is a Sharp needle covered by a grey membrane. PLEASE do not touch this!
7- Mix the lactulose substrate in approximately 8oz of water. Do not drink it until after the first breath is collected.

It is important not to retest too soon after antibiotic treatment or any sibo treatment for that matter. Wait a minimum of 30 days before retesting, >Click here to find out why<

Veuillez cliquer ici pour les formulaires de test d'haleine française

This breath test is the primary method of SIBO testing for SIBO CLINIC CANADA. 

No Doctor or Practitioner referrals required. Anyone is free to order the SIBO Breath test from SIBO Clinic Canada. 

Shipping policy:

International orders > please contact us directly,  info@sibocliniccanada.com


Lactulose, unlike glucose, is not absorbed in your system. It is a sugar not digested by the body, meaning that it is able to travel all the way through the small intestine without modification in normal physiology. The lactulose breath test thus allows detection of abnormally increased numbers of bacteria located in the distal small intestine. It acts as a food source for bacteria, if present in the small intestine. If bacteria are present, lactulose will allow the bacteria to ferment, producing hydrogen (H2) and/or methane (CH4). These gases are diffused into the bloodstream and exhaled by the breath.  

With lactulose testing, breath samples are collected every 20 minutes over the course of 3 hours.

SIBO CLINIC CANADA uses the gold standard Quintron BreathTracker for analyzing the breath samples that are returned to our facility.  We use extensive quality control, which includes carbon dioxide measurements, to ensure an acceptable air sample.  We comply with the highest standards of excellence.


Anyone suffering from symptoms of stomach distension, bloating, gas, diarrhea/constipation, indigestion and IBS can benefit from this testing. Consider this breath test the first step to understanding your condition and treatment options.


SIBO can be debilitating. If you suffer from any of the above symptoms, chances are it is greatly affecting your daily life. Left unchecked, SIBO can lead to any of the following:

Alzheimer’s, Parkinson’s, Multiple Sclerosis, Acid reflux, Fibromyalgia, Chronic Fatigue Syndrome, Inflammatory diseases, Irritable Bowel Syndrome, Type 2 Diabetes, High Blood Pressure, Brain Fog, Autoimmune Disease, Leaky Gut, Acne, Restless Leg Syndrome, Weigh loss/Weight gain and many other conditions. 


Dietary carbohydrates, unabsorbed in the small intestine, are fermented in the large intestine by bacteria, resulting in the production of hydrogen and/or methane gases.

In patients with SIBO, bacterial fermentation of these carbohydrates occurs in the small intestine, producing gas which then gets absorbed into the bloodstream and exhaled in the breath.


The lactulose breath test is a very simple, non-invasive breath test that you can do in the comfort of your home. You must follow a 24 or 48 hour preparation diet, depending on how severe your symptoms are prior to performing the test.

The step-by-step guidelines will ensure proper results. If the preparation guidelines are not followed, we cannot guarantee the accuracy of your test results.

Take the following actions as preparation steps to improve breath test accuracy:

  • Two weeks before the test: finish any antimicrobials (drugs, botanicals, or nutrients), is not having any atypical diarrhea, and will not receive a colonoscopy or barium enema before the test.
  • One day before the test: avoid all laxatives, high-dose vitamin C, and magnesium supplements.
  • One to two days before the test: avoid all high-fiber and lactose-containing foods, condiments, spices, and herbs. Follow the. Consume only meat, fish, plain steamed jasmine rice, eggs, and clear meat broth. Limit fat and oils, salt and pepper, and weak black coffee and tea. Stop all non-essential medication.
  • Fast 12 hours before the test, if you are testing first thing in the morning please be awake at least one hour prior to the test. PLEASE avoid smoking and vigorous exercise.
  • Brush teeth and try to rinse with an antiseptic. The antiseptic will ensure that oral bacteria is gone as to not have a higher than normal baseline value. This is not absolutely necessary. 

If you are unsure about any of the preparation instructions, please do not hesitate to contact us at info@sibocliniccanada.com or call 506-381-9847.

Inside the testing kit you will find:

  • breath collection device
  • 10 tubes
  • the substrate (Lactulose) Mix in 6 or 8 oz of water
  • intake form (please fill out and sign)
  • labels for each tube
  • extra bubble wrap envelope for return to the testing facility 
  • return instructions 

Please read all instructions carefully before performing the test. 

Please allow 24 hours (48, if constipated) before you start the test. Follow the diet guide and consume only the foods listed. If you are vegan or vegetarian, you may consume small amounts of tofu or soaked lentils. 

Please keep in mind that:

SIBO CLINIC CANADA does not diagnose or treat SIBO, this is not a diagnoses. Any diagnoses or treatment should be made by your healthcare practitioner. The SIBO Breath test is a guideline only that is meant to aid your practitioner in their diagnosis, which is made in accordance with other clinical data that is unavailable to SIBO CLINIC CANADA. It is important to work with your healthcare provider to determine an appropriate treatment plan based on an individual's symptoms and test results.  

Watch the video on how to perform the breath test.